abstract：:We have studied the differentiation of the oocyte vitelline coat (VC) and jelly coat (JC) of the starfish, Astropecten aurantiacus. The precursor material of both envelopes is secreted by the oocyte while the follicle cells do not appear to participate in the secretory process. The first indication of differentiation ...

journal_title：Developmental biology

authors： Santella L,Monroy A,Rosati F

更新日期：1983-10-01 00:00:00

abstract：:A careful reanalysis of the literature indicates that the initial mechanism of activation in sea urchin eggs is remarkably similar to the mechanism established in medaka eggs: i.e., sea urchin eggs are activated by a qualitatively and quantitatively similar calcium explosion; one which is propagated in a wave sustaine...

journal_title：Developmental biology

authors： Jaffe LF

更新日期：1983-10-01 00:00:00

abstract：:We have analyzed several aspects of the development of flies carrying mutations at the Glued locus. Optic lobe abnormalities in individuals heterozygous for the original Glued allele were previously shown to result from an action of this mutation in the retinula cells. We have estimated when the functioning of this ge...

journal_title：Developmental biology

authors： Harte PJ,Kankel DR

更新日期：1983-09-01 00:00:00

abstract：:Procedures are described for removal of the vitelline membrane from gently fixed Drosophila embryos en masse. The resulting embryos retain excellent structural integrity and are now suitable for a variety of immunocytochemical and biochemical characterizations. ...

journal_title：Developmental biology

authors： Mitchison TJ,Sedat J

更新日期：1983-09-01 00:00:00

abstract：:We have used an in vitro model to investigate the relationship between cell proliferation, cell maturation, and intercellular junctional changes that are seen during normal liver development. Chick liver fragments of embryonic stages 21, 28, and 39 were grown in organ culture in Medium 199 with 10% bovine serum albumi...

journal_title：Developmental biology

authors： Meyer RA,Overton J

更新日期：1983-09-01 00:00:00

abstract：:Previous experiments in which vitamin A has been administered to developing or regenerating limbs have shown that different limb axes are affected. In regenerating axolotl limbs, serial reduplications in the proximodistal axis are produced. In the developing chick limb bud, mirror-imaged reduplications in the anteropo...

journal_title：Developmental biology

authors： Maden M

更新日期：1983-08-01 00:00:00

abstract：:The structure of the three polypeptide chains of the laminin subunits and the number of glycosylation sites in each polypeptide chain were determined using peptide mapping by high-performance liquid chromatography. Analysis of the [35S]methionine-labeled underglycosylated laminin isolated from tunicamycin (TM)-treated...

journal_title：Developmental biology

authors： Howe CC,Dietzschold B

更新日期：1983-08-01 00:00:00

abstract：:The level of early histone mRNA in Strongylocentrotus purpuratus changes abruptly at 6 hr of development, increasing an average of 10-fold by 9-10.5 hr and then decreasing over 2-fold by 13.5-15 hr. These changes occur when the late embryonic mRNA is still a very minor component of histone gene transcription. The exac...

journal_title：Developmental biology

authors： Weinberg ES,Hendricks MB,Hemminki K,Kuwabara PE,Farrelly LA

更新日期：1983-07-01 00:00:00

abstract：:Primary cultures of cardiac myocytes from newborn normal and genetically cardiomyopathic (strain UM-X7.1) hamsters were analyzed by electron microscopy and immunofluorescent staining for myosin, actin, tropomyosin, and alpha-actinin. Antibody staining of these contractile proteins demonstrates that both normal and car...

journal_title：Developmental biology

authors： Lemanski LF,Tu ZH

更新日期：1983-06-01 00:00:00

abstract：:A new method is presented for preparation of highly purified plasma membranes from fertilized sea urchin eggs. The purified plasma membranes are in vesicle form and are highly enriched in ouabain inhibitable, Na+/K+ ATPase activity. Analysis of membrane proteins by sodium dodecyl sulfate-gel electrophoresis indicates ...

journal_title：Developmental biology

authors： Ribot H,Decker SJ,Kinsey WH

更新日期：1983-06-01 00:00:00

abstract：:Nontransformed cultures of vascular smooth muscle cells proliferate until they form a confluent sheet of cells. Subsequently, the cells become reorganized to form multicellular nodules that are loosely attached to the substrate. The formation of nodules is facilitated by the addition of medium conditioned by nodular c...

journal_title：Developmental biology

authors： Brennan MJ,Millis AJ,Mann D,Fritz KE

更新日期：1983-06-01 00:00:00

abstract：:Differences are demonstrated in the chondrogenic potential of cells derived from the distal and proximal halves of chick wing buds from as early as stage 23, prior to the appearance of overt cartilage differentiation. In high cell density cultures, cells obtained from the distal portions of stage 23 or 24 limb buds ar...

journal_title：Developmental biology

authors： Swalla BJ,Owens EM,Linsenmayer TF,Solursh M

更新日期：1983-05-01 00:00:00

abstract：:The pattern of innervation in 13 chicken hindlimb muscles was studied at various stages of development in order to examine the mechanisms which regulate its formation. The pattern of innervation was visualized by examining the distribution of fiber types within each muscle. It was found that the fiber type which a myo...

journal_title：Developmental biology

authors： McLennan IS

更新日期：1983-05-01 00:00:00

abstract：:Concentration of mast-cell precursors in hematopoietic tissues of mouse embryos was evaluated by a limiting dilution method. Cells from yolk sacs, livers, and bodies of (WB x C57BL/6)F1 (hereafter called WBB6F1)- +/+ embryos were injected directly into the skin of adult WBB6F1-W/Wv mice which were genetically depleted...

journal_title：Developmental biology

authors： Sonoda T,Hayashi C,Kitamura Y

更新日期：1983-05-01 00:00:00

abstract：:The polypeptide labeling patterns of somatic cells, gonidia (asexual reproductive cells), embryos, and juvenile spheroids of Volvox carteri cultures synchronized by a light/dark cycle were studied as a function of developmental stage and incubation condition. Specimens were exposed to 35SO=4 for 1-hr periods at select...

journal_title：Developmental biology

authors： Kirk DL,Kirk MM

更新日期：1983-04-01 00:00:00

abstract：:We have previously reported the detection of cAMP waves within monolayers of aggregating Dictyostelium discoideum cells (K. J. Tomchik and P.N. Devreotes, 1981, Science 212, 443-446). The computer-assisted analysis presented here of the fluorographic images of the cAMP waves reveals (1) all the waves have a consistent...

journal_title：Developmental biology

authors： Devreotes PN,Potel MJ,MacKay SA

更新日期：1983-04-01 00:00:00

abstract：:The origin and development of mouse kidney vasculature were examined in chorioallantoic grafts of early kidney rudiments and of experimentally induced explants of separated metanephric mesenchymes. Whole kidney rudiments developed into advanced stages, expressed the segment-specific antigenic markers of tubules and th...

journal_title：Developmental biology

authors： Sariola H,Ekblom P,Lehtonen E,Saxén L

更新日期：1983-04-01 00:00:00

abstract：:The morphology of skeletal tissues formed in each of the branchial arches of higher vertebrates is unique. In addition to these structures, which are derived from the neural crest, the crest-derived connective tissues and mesodermal muscles also form different patterns in each of the branchial arches. The objective of...

journal_title：Developmental biology

authors： Noden DM

更新日期：1983-03-01 00:00:00

abstract：:Chondrocytes were isolated from the auricular cartilage of rabbits, aged 1 week to 30 months, and grown in short-term cell culture. The cells from the 1-week animals were small, polygonal, and mononucleated, while the chondrocytes from the older animals were larger, rounded, and frequently binucleated. The synthesis o...

journal_title：Developmental biology

authors： Madsen K,Moskalewski S,von der Mark K,Friberg U

更新日期：1983-03-01 00:00:00

abstract：:A group of proteins judged on several criteria to be yolk proteins have been isolated from a homogenate of the nematode Caenorhabditis elegans. Comparison of partial proteolysis fragments indicates that the two bands of a 170,000-dalton doublet (yp170) are closely related; bands observed at 115,000 daltons (yp115) and...

journal_title：Developmental biology

authors： Sharrock WJ

更新日期：1983-03-01 00:00:00

abstract：:We present evidence to show that differentiation in wing cells to produce hairs is synchronous over the distal 90% of the wing surface (approximately 28,000 cells). In spite of this synchrony within such a large area a temporal gradient exists between zones (in general anterior to posterior) on the animal surface with...

journal_title：Developmental biology

authors： Mitchell HK,Petersen NS

更新日期：1983-02-01 00:00:00

abstract：:We have studied the effect of saline and acid extracts of chick brain on the total number of acetylcholine (ACh) receptors and the number of receptor clusters in cultured chick muscle cells. Myotubes in 7-day cultures responded more rapidly to brain extract than did myotubes in 4-day cultures, so the older cells were ...

journal_title：Developmental biology

authors： Buc-Caron MH,Nystrom P,Fischbach GD

更新日期：1983-02-01 00:00:00

abstract：:A cDNA library was used to measure changes in many individual mRNAs during muscle differentiation in culture. A library of 1000 clones was constructed from total myofiber poly(A) RNA. About 23% of these clones gave a detectable colony hybridization signal using end-labeled myofiber mRNA, the remainder containing muscl...

journal_title：Developmental biology

authors： Devlin RB,Haskell SG

更新日期：1983-02-01 00:00:00

abstract：:The changes in the intracellular pH (pHi) of sea urchin sperm associated with motility initiation and acrosome reaction were investigated using uptake of two different probes; 9-aminoacridine and methylamine, as a qualitative index. Sperm suspended in Na+-free sea water were immotile and able to concentrate these amin...

journal_title：Developmental biology

authors： Lee HC,Johnson C,Epel D

更新日期：1983-01-01 00:00:00

abstract：:The rabbit sperm membrane autoantigen RSA-1 is a sialoglycoprotein of 13,000 daltons which first appears on the surface of pachytene spermatocytes. Using specific antiserum to RSA-1 the antigen has been localized by immunofluorescence and immunoperoxidase staining. On testicular cells labeled at 37 degrees C, RSA-1 is...

journal_title：Developmental biology

authors： O'Rand MG,Romrell LJ

更新日期：1981-06-01 00:00:00

abstract：:We have compared insulin binding by heart cells at 7 and 14 days of development. Species specificity, optimum pH, temperature relationships, and time to equilibrium for binding of insulin were the same in both 7 and 14-day systems. Curvilinear Scatchard plots for chicken insulin binding were demonstrated. Binding affi...

journal_title：Developmental biology

authors： Serravezza JC,Wheeler FB,DeHaan RL,Elsas LJ

更新日期：1981-06-01 00:00:00

abstract：:We report periodic movements during erection of Dictyostelium discoideum (Dd) sorocarps. Our observations lead to the working hypothesis that Dd sorocarp erection occurs by two superimposed processes: one periodic, with a modal period of 6 1/2 min, and one continuous. We tentatively identify the periodic process with ...

journal_title：Developmental biology

authors： Durston AJ,Cohen MH,Drage DJ,Potel MJ,Robertson A,Wonio D

更新日期：1976-09-01 00:00:00

abstract：:Eggs and embryos of the sea urchin Lytechinus pictus were labeled with [35S]methionine. Aqueous extracts of protein were prepared and analyzed by a high resolution two-dimensional polyacrylamide gel electrophoresis system described recently by O'Farrell utilizing isoelectric focusing and sodium dodecyl sulfate electro...

journal_title：Developmental biology

authors： Brandhorst BP

更新日期：1976-09-01 00:00:00